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    Structured Review

    GenScript corporation fp9-peg12-biotin
    KEY RESOURCES TABLE
    Fp9 Peg12 Biotin, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fp9-peg12-biotin/fp9+peg12+biotin/pmc11003769-265-7-8
    Average 90 stars, based on 1 article reviews
    fp9-peg12-biotin - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution"

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.113948

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Staining, Virus, Mutagenesis, Clinical Proteomics, Recombinant, Transfection, Reporter Gene Assay, Sequencing, Expressing, Plasmid Preparation, Software

    Related Articles

    Blocking Assay:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024

    Incubation:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024

    Cell Culture:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024

    Staining:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024

    Virus:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024

    Mutagenesis:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024

    Clinical Proteomics:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024

    Recombinant:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024

    Transfection:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024

    Reporter Gene Assay:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024

    Sequencing:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024

    Expressing:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024

    Plasmid Preparation:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024

    Software:

    Article Title: Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization
    Article Snippet: FP9-PEG12-biotin , Genscript , N/A.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution
    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies LIVE/DEADTM Fixable Violet Stain ThermoFisher Scientific Cat# L34955 Anti-human CD3 BV421 BD Biosciences Cat# 562877; RRID:AB_2737860 Anti-human CD4 BV421 BioLegend Cat# 317433; RRID:AB_11150413 Anti-human CD8 BV421 BioLegend Cat# 301036; RRID:AB_10898322 Anti-human CD14 BV421 BioLegend Cat# 301830; RRID:AB_10959324 Anti-human CD20 PerCP/Cy5.5 BioLegend Cat# 302326; RRID:AB_893285 Anti-Human IgM FITC BD Biosciences Cat# 562029; RRID:AB_10895795 Anti-Human IgG A700 BD Biosciences Cat# 561296; RRID:AB_10612406 FP9 PE This paper N/A FP9 APC-Cy7 This paper N/A DFPH-a.01 Kong et al.10 N/A Mouse anti-human IgG BD Biosciences Cat# 555784; RRID:AB_396119 FP-specific mAbs from immunized NHP Kong et al.10 and this paper N/A Bacterial and virus strains BG505 N611Q mutant Kong et al.14 N/A HIV-1 wild-type strains Kong et al.14 N/A Biological samples NHP plasma and PBMCs Kong et al.10 N/A Chemicals, peptides, and recombinant proteins FP9-PEG12-biotin Genscript N/A Mouse Anti-Monkey IgG-HRP SouthernBiotech Cat# 4700-05 Streptavidin, R-PE conjugate ThermoFisher Scientific Cat# S21388 Streptavidin, APC-Cy7 conjugate BD Biosciences Cat# 554063 Streptavidin VWR Cat# 97062-808 SureBlue TMB Microwell Peroxidase Substrate VWR Cat# 95059-286 DEAE-Dextran hydrochloride Sigma Aldrich Cat# D9885-10G Human IL-2 This paper N/A Human IL-21 ThermoFisher Scientific Cat# PHC0213 Human IL-4 Miltenyi Biotec Cat# 130-093-922 Human BAFF GenScript Cat# Z02976-1 HIV-1 BG505.DS.SOSIP.S613A Env trimer This paper N/A Critical commercial assays PEI 25K transfection reagent Polysciences Cat#23966-1 CTS Opti-MEM I Medium Fisher Scientific Cat# A4124802 Steadylite plus Reporter Gene Assay System PerkinElmer Cat# 6066751 Deposited data Sequence of antibodies This paper GenBank: OR576798-OR576809 Experimental models: cell lines 3T3muCD40L cells NIH/VRC N/A TZM-bl cells NIH AIDS Reagent Program Cat# 8129 Expi293 cells ThermoFisher Scientific Inc Cat# A14527 Experimental models: organisms/strains Indian origin rhesus macaque Kong et al.10 N/A (Continued on next page) 14 Cell Reports 43, 113948, March 26, 2024



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    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Frequency-potency analysis of IgG+ memory B cells delineates neutralizing antibody responses at single-cell resolution

    doi: 10.1016/j.celrep.2024.113948

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: After blocking, plates were first incubated with FP9-PEG12-biotin (Genscript, Piscataway, NJ) at 0.4 μM for 1 h at 37°C, then followed with cell culture supernatant at a 1:5 dilution for another hour at 37°C.

    Techniques: Staining, Virus, Mutagenesis, Clinical Proteomics, Recombinant, Transfection, Reporter Gene Assay, Sequencing, Expressing, Plasmid Preparation, Software